scrna-seq-pipeline

Scanpy-based single-cell RNA-seq analysis pipeline covering loading (10X, h5ad), QC, normalization, HVG selection, PCA/UMAP, Leiden clustering, differential expression, and batch correction (Harmony, scVI). Use when the user mentions single-cell, scRNA-seq, Scanpy, AnnData, UMAP, clustering, 10X, h5ad, leiden, highly variable genes, Harmony, scVI, cluster cells, find marker genes, filter low-quality cells, gene…